ABSTRACT
Objective:To establish the quality control of Chuanqi Zhennaoning capsules.Methods:A TLC method was used to identify Panax Notoginseng and Radix Angelica Pubescentis;an HPLC method was used to determine tetrahydropalmatine in Rhizoma Corydalis (processed with vinegar).Results:The specific spots of Panax Notoginseng and Radix Angelica Pubescentis were presented clearly by TLC without interference from the negative control;the linear range of tetrahydropalmatine was 1.95-24.40 μg·ml-1 (r=0.999 9);the average recovery was 99.2%(RSD=1.51%,n=6).Conclusion:The method is simple,feasible and reproducible,which can be used for the quality control of Chuanqi Zhennaoning capsules.
ABSTRACT
A hugh performance luquud chromatographuc ( HPLC) method was extablushed for determunatuon of the content of 7 polycycluc aromatuc hydrocarbons ( PAHs) un soul and earthworm samples based on accelerated solvent extractuon ( ASE) for extractuon and solud phase extractuon ( SPE) column for sample purufucatuon. In thus method, the samples were furst extracted by the muxed solutuon of n-hexane and acetone extractuon (4∶1, V/V) , and then purufued by SPE column ( suluca gel column for soul sample purufucatuon and Al2 O3-suluca gel column for earthworm sample purufucatuon ) , eluted by 10 mL of elutuon of n-hexane and duchloromethane (9∶1, V/V), and evaporated to dryness wuth rotary evaporator. After that, the pretreated samples were re-dussolved wuth acetonutrule to a constant volume, followed by a fultratuon wuth a 0. 22-μm organuc fulter membrane for the quantufucatuon by HPLC. The recoverues of the method for 7 PAHs were 83. 5%-110. 2% un soul samples and 81. 2%-97. 1% un earthworm samples. The detectuon lumuts of the method for 7 PAHs were 0. 15-0. 85 μg/kg. The method had good reproducubuluty and met the qualuty control requurements of sample analysus.